Specificity in immunocytochemical staining.
نویسندگان
چکیده
منابع مشابه
Immunocytochemical staining of T and B lymphocytes in serous effusions.
Cell smears from serous effusions containing large numbers of lymphoid cells were stained by the alkaline phosphatase-anti-alkaline phosphatase technique with a panel of monoclonal antibodies, including anti-B and anti-T cell antibodies and anti-HLA-DR. Samples from 17 patients with lymphoproliferative disorders--such as chronic lymphocytic leukaemia and non-Hodgkin's lymphoma--and from 19 pati...
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The sensitivity and specificity of immunocytochemical staining of mycobacterial antigens in the cytoplasm of cerebrospinal fluid (CSF) macrophages for diagnosis of tuberculous meningitis (TBM) was prospectively compared with Ahuja criteria from 393 consecutive CSF specimens. The assay can play an important role for the diagnosis of TBM, with sensitivity of 73.5% and specificity of 90.7%.
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AIMS/HYPOTHESIS Protein gene product 9.5 (PGP 9.5) is a marker for neuroendocrine cells but has not been used for pancreatic islet cells and pancreatic endocrine tumors (PETs). Antibodies for PGP 9.5 are now commercially available for immunocytochemical study, with which immunostaining may be able to differentiate between benign and malignant PETs. RESULTS All 4 kinds of normal islet cells we...
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introduction: the present study was undertaken to produce alkaline phosphatase anti- alkaline phosphatase (apaap) complex and to use it in one of the most practical methods of determining the site of antigen in tissue or in cell, i.e. apaap technique, and its comparison with similar foreign products. materials and methods: in this basic and applied study the secreted antibodies of the two hybri...
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Antibodies against gamma-aminobutyric acid (GABA)-glutaraldehyde-lysine were obtained by using a procedure based upon (i) a high yield of coupling of GABA to protein carriers, (ii) the reduction of the resulting immunoreactive double bonds, and (iii) a protocol of alternative immunizations using different immunogens having in common only the GABA-glutaraldehyde-lysine segment. This strategy led...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1976
ISSN: 0022-1554,1551-5044
DOI: 10.1177/24.10.789759